Metatranscriptomic and metagenomic description of the bacterial nitrogen metabolism in waste water wet oxidation effluents

Crovadore, Julien (Haute école du paysage, d’ingénierie et d’architecture de Genève (hepia), HES-SO // Haute Ecole Spécialisée de Suisse Occidentale ; Institut Terre, Nature et Environnement (inTNE)) ; Soljan, Vice (Puratis Sàrl ; EPFL Innovation Park, Building C, 1015 Lausanne, Switzerland) ; Calmin, Gautier (HES-SO // Haute Ecole Spécialisée de Suisse Occidentale ; domaine ingénierie et architecture) ; Chablais, Romain (Haute école du paysage, d’ingénierie et d’architecture de Genève (hepia), HES-SO // Haute Ecole Spécialisée de Suisse Occidentale ; Institut Terre, Nature et Environnement (inTNE)) ; Cochard, Bastien (Haute école du paysage, d’ingénierie et d’architecture de Genève (hepia), HES-SO // Haute Ecole Spécialisée de Suisse Occidentale ; Institut Terre, Nature et Environnement (inTNE)) ; Lefort, François (Haute école du paysage, d’ingénierie et d’architecture de Genève (hepia), HES-SO // Haute Ecole Spécialisée de Suisse Occidentale ; Institut Terre, Nature et Environnement (inTNE))

Anaerobic digestion is a common method for reducing the amount of sludge solids in used waters and enabling biogas production. The wet oxidation process (WOX) improves anaerobic digestion by converting carbon into methane through oxidation of organic compounds. WOX produces effluents rich in ammonia, which must be removed to maintain the activity of methanogens. Ammonia removal from WOX could be biologically operated by aerobic granules. To this end, granulation experiments were conducted in 2 bioreactors containing an activated sludge (AS). For the first time, the dynamics of the microbial community structure and the expression levels of 7 enzymes of the nitrogen metabolism in such active microbial communities were followed in regard to time by metagenomics and metatranscriptomics. It was shown that bacterial communities adapt to the wet oxidation effluent by increasing the expression level of the nitrogen metabolism, suggesting that these biological activities could be a less costly alternative for the elimination of ammonia, resulting in a reduction of the use of chemicals and energy consumption in sewage plants. This study reached a strong sequencing depth (from 4.4 to7.6Gb) and enlightened a yet unknown diversity of the microorganisms involved in the nitrogen pathway. Moreover, this approach revealed the abundance and expression levels of specialised enzymes involved in nitrification, denitrification, ammonification, dissimilatory nitrate reduction to ammonium (DNRA) and nitrogen fixation processes in AS.


Mots-clés:
Type d'article:
scientifique
Faculté:
Ingénierie et Architecture
Filière:
Chimie
Ecole:
HEPIA Haute école du paysage, d’ingénierie et d’architecture de Genève
Classification:
Chimie et science de la vie
Ingénierie
Autres
Date:
2017
Titre du document hôte:
Heliyon
Numérotation (vol. no.):
October 2017, vol. 3, no. 10
DOI:
ISSN:
2405-8440
Le document apparaît dans:

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 Notice créée le 2017-11-14, modifiée le 2017-11-14

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