Electrospinning of two bio-sourced polymers, suitable for edible food packaging applications, chitosan, as well as pullulan, is investigated in view of large area fiber mat production. The fiber shape and dimensions were measured using scanning electron microscopy and atomic force microscopy, for various process parameters using rotating disc electrodes. An electrospinning pilot production system was designed for the fabrication of large area nanofiber and microfiber mats, using a drum collector electrode together with controllable lateral needle motion. System tests were performed using polyethylene oxide. Finally, large area fiber mats of pullulan were produced. The system is promising for the development of edible, bio-sourced packaging applications.