Background and aims: While a causal relationship between pain- related fear and spinal movement avoidance in patients with chronic low back pain (CLBP) has frequently been postulated, evidence supporting this relationship is limited. This study aimed to test if decreases in pain- related fear or catastrophizing were associated with improvements in spinal biomechanics, accounting for possible changes in movement-evoked pain. Methods: Sixty-two patients with CLBP were assessed before and after an interdisciplinary rehabilitation program (IRP). Pain- related fear was assessed with general and task-specific measures. Lower and upper lumbar angular amplitude and velocity as well as paraspinal muscle activity were recorded during five daily-life tasks to evaluate spinal biomechanics. Relationships were tested with multivariable linear regression analyses. Results: The large decreases in pain- related fear and catastrophizing following the IRP were scarcely and inconsistently associated with changes in spinal biomechanics (< 3% of the models reported a statistically significant association). Results remained comparable for activities inducing more or less fear, for specific or general measures of pain- related fear, and for analyses performed on the entire population or limited to subgroups of patients with higher levels of task-specific fear. In contrast, reductions in task-specific pain- related fear were significantly associated with decreases in movement-evoked pain in all tasks (r= 0.26– 0.62, p ≤ 0.02).Conclusion: This study does not support an association between pain- related fear and spinal movement avoidance. However, it provides evidence supporting a direct relationship between decreased pain- related fear and decreased movement-evoked pain, possibly explaining some mechanisms of the rehabilitation programs.