Synthesis, characterization and biosensing-applications of gold nanoparticles : experiments for photonics and other nonchemistry curricula in the higher education of engineers

Bi, Hongyan (School of Management and Engineering Vaud, HES-SO // University of Applied Sciences Western Switzerland ; College of Food Science and Engineering, Shanghai Ocean University, Hucheng Ring Road 999, Pudong New District, 201306 Shanghai, China) ; Schintke, Silvia (School of Management and Engineering Vaud, HES-SO // University of Applied Sciences Western Switzerland)

With the development of nanoscience and nanotechnology, the application of nanoparticles is getting broader and interdisciplinary. For preparing engineering students to work in the increasingly interdisciplinary sectors of photonics, materials engineering, nanotechnologies, or health engineering, laboratory curricula or term project training in applied nanoscience is increasingly required. This article presents laboratory experiments for the higher education of photonics and other non-chemistry graduate students (postgraduates) or even undergraduates to be basically skilled with the synthesis, characterization and bio-applications of gold nanoparticles (Au NPs). The presented laboratory training experiments are based on low investment costs of instrumentation. The entire chain is illustrated from conventional reaction principles for Au NP synthesis, over optical characterization to practical operation in optical biosensors developments. Using myoglobin as a model protein, UV/vis absorbance experiments illustrate how to evaluate the amount of proteins that are adsorbed to the NPs. Potentially the synthesized NPs can be used to extract low-abundant proteins for bio-sensing application, or to remove excess proteins in samples with complicated background like sera. The designed experiments are suitable for laboratory project periods of two days, and extendable to weeks, or also to term projects under minor alteration.


Keywords:
Conference Type:
short paper
Faculty:
Ingénierie et Architecture
School:
HEIG-VD
Institute:
COMATEC - Institut de Conception, Matériaux, Emballage & Conditionnement
Subject(s):
Ingénierie
Publisher:
Bucharest, Romania, 1-5 July 2018
Date:
2018-07
Bucharest, Romania
1-5 July 2018
Pagination:
4 p.
Published in:
Proceedings of the 20th International Conference on Transparent Optical Networks, Bucharest, Romania, 1-5 July 2018
DOI:
ISSN:
2161-2064
ISBN:
978-1-5386-6605-0
External resources:
Appears in Collection:

Note: The status of this file is: restricted


 Record created 2018-10-09, last modified 2018-12-20

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