Computational prediction of inter-species relationships through omics data analysis and machine learning

Carvalho Leite, Diogo Manuel (School of Management and Engineering Vaud, HES-SO // University of Applied Sciences Western Switzerland ; SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland) ; Brochet, Xavier (School of Management and Engineering Vaud, HES-SO // University of Applied Sciences Western Switzerland ; SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland) ; Resch, Gregory (Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland) ; Que, Yok-Ai (Department of Intensive Care Medicine, Bern University Hospital (Inselspital), Bern, Switzerland) ; Neves, Aitana (SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland) ; Peña-Reyes, Carlos (School of Management and Engineering Vaud, HES-SO // University of Applied Sciences Western Switzerland ; SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland)

Background: Antibiotic resistance and its rapid dissemination around the world threaten the efficacy of currently-used medical treatments and call for novel, innovative approaches to manage multi-drug resistant infections. Phage therapy, i.e., the use of viruses (phages) to specifically infect and kill bacteria during their life cycle, is one of the most promising alternatives to antibiotics. It is based on the correct matching between a target pathogenic bacteria and the therapeutic phage. Nevertheless, correctly matching them is a major challenge. Currently, there is no systematic method to efficiently predict whether phage-bacterium interactions exist and these pairs must be empirically tested in laboratory. Herein, we present our approach for developing a computational model able to predict whether a given phage-bacterium pair can interact based on their genome. Results: Based on public data from GenBank and phagesDB.org, we collected more than a thousand positive phage-bacterium interactions with their complete genomes. In addition, we generated putative negative (i.e., non-interacting) pairs. We extracted, from the collected genomes, a set of informative features based on the distribution of predictive protein-protein interactions and on their primary structure (e.g. amino-acid frequency, molecular weight and chemical composition of each protein). With these features, we generated multiple candidate datasets to train our algorithms. On this base, we built predictive models exhibiting predictive performance of around 90% in terms of F1-score, sensitivity, specificity, and accuracy, obtained on the test set with 10-fold cross-validation. Conclusion: These promising results reinforce the hypothesis that machine learning techniques may produce highly-predictive models accelerating the search of interacting phage-bacteria pairs.


Keywords:
Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEIG-VD
Institute:
IICT - Institut des Technologies de l'Information et de la Communication
Subject(s):
Ingénierie
Date:
2018-11
Pagination:
9 p.
Published in:
BMC Bioinformatics
Numeration (vol. no.):
2018, vol. 19 (supp. 14), no. 420, pp. 151-159
DOI:
ISSN:
1471-2105
Appears in Collection:



 Record created 2018-12-04, last modified 2018-12-20

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