Soluble and solid iron reduction assays with Desulfitobacterium hafniense

Comensoli, Lucrezia (University of Neuchâtel, Switzerland) ; Maillard, Julien (Ecole Polytechnique Fédérale de Lausanne, Switzerland) ; Kooli, Wafa M. (University of Neuchâtel, Switzerland) ; Junier, Pilar (University of Neuchâtel, Switzerland) ; Joseph, Edith (Haute Ecole Arc Conservation-Restauration, HES-SO University of Applied Sciences and arts Western Switzerland ; University of Neuchâtel, Switzerland)

There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict anaerobe Desulfitobacterium hafniense (strains TCE1 and LBE). We investigated iron reduction using three different Fe(III) sources: Fe(III)-citrate (a soluble phase), akaganeite (solid iron phase), and corroded coupons. This protocol describes a method that combines spectrophotometric quantification of the complex Fe(II)-Ferrozine® with mineral characterization by scanning electron microscopy and Raman spectroscopy. These three methods allow assessing reductive dissolution of ferric iron and biogenic mineral production as a promising alternative for the development of an innovative sustainable method for the stabilization of corroded iron.


Keywords:
Article Type:
scientifique
Faculty:
Design et Arts visuels
School:
HE-Arc CR
Institute:
Unité de recherche en Conservation-restauration de la Haute Ecole Arc - UR-ARC CR
Subject(s):
Conservation - Restauration
Date:
2018-09
Published in:
Bio-protocol
Numeration (vol. no.):
2018, Vol. 8, iss 17
DOI:
ISSN:
2331-8325
Appears in Collection:

Note: The status of this file is: restricted


 Record created 2019-05-13, last modified 2019-05-13

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