Continuous micro-production using enzymatic reaction and online monitoring

Moser, Regula (School of Engineering and Architecture (HEIA-FR), HES-SO // University of Applied Sciences Western Switzerland) ; Chappuis, Thierry (School of Engineering and Architecture (HEIA-FR), HES-SO // University of Applied Sciences Western Switzerland) ; Vanoli, Ennio (School of Engineering and Architecture (HEIA-FR), HES-SO // University of Applied Sciences Western Switzerland) ; Crelier, Simon (School of Engineering and Architecture (HEIA-FR), HES-SO // University of Applied Sciences Western Switzerland) ; Naef, Olivier (School of Engineering and Architecture (HEIA-FR), HES-SO // University of Applied Sciences Western Switzerland)

A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit® C, controlled-pore glass (CPG), and Sepharose.


Keywords:
Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEIA-FR
Institute:
ChemTech - Institut des technologies chimiques
Date:
2010-11
Pagination:
4 p.
Published in:
CHIMIA International Journal for Chemistry
Numeration (vol. no.):
2010, vol. 64, no. 11, pp. 799-802
DOI:
ISSN:
0009-4293
Appears in Collection:



 Record created 2019-10-29, last modified 2019-10-29

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