Continuous micro-production using enzymatic reaction and online monitoring
Moser, Regula; Chappuis, Thierry; Vanoli, Ennio; Crelier, Simon; Naef, Olivier
2010

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Abstract

A micro-reactor coupled to a microfluidic system and an online UV/VIS spectrometer is described. The enzymatic reaction studied is the hydrolysis of the N-benzoyl-L-tyrosine ethyl ester (BTEE) to N-benzoyl-L-tyrosine (BT) and ethanol, catalyzed by chymotrypsin. The production is online monitored with UV spectroscopy at 256 nm. Three different immobilization methods of the enzyme are discussed: Eupergit® C, controlled-pore glass (CPG), and Sepharose.

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Title Continuous micro-production using enzymatic reaction and online monitoring
Author(s) Moser, Regula (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Chappuis, Thierry (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Vanoli, Ennio (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Crelier, Simon (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Naef, Olivier (School of Engineering and Architecture (HEIA-FR), HES-SO University of Applied Sciences Western Switzerland)
Date 2010-11
Published in CHIMIA International Journal for Chemistry
Volume 2010, vol. 64, no. 11, pp. 799-802
Pagination 4 p.
DOI https://doi.org/10.2533/chimia.2010.799
ISSN 0009-4293
Keywords chymotrypsin ; enzymatic reaction ; enzyme immobilization ; micro-production ; micro-reactor
Article Type scientifique
Faculty Ingénierie et Architecture
School HEIA-FR
Institute ChemTech - Institut des technologies chimiques
Record Appears in Scientific Articles
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