Anaerobic production of poly(3-hydroxybutyrate) and its precursor 3-hydroxybutyrate from synthesis gas by autotrophic clostridia

Flüchter, Sebastian (Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany) ; Follonier, Stéphanie (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland) ; Schiel-Bengelsdorf, Bettina (Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany) ; Bengelsdorf, Frank R. (Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany) ; Zinn, Manfred (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland) ; Dürre, Peter (Institut für Mikrobiologie und Biotechnologie, Universität Ulm, Ulm, Germany)

Anaerobic production of the biopolymer poly(3-hydroxybutyrate) (PHB) and the monomer 3-hydroxybutyrate (3-HB) was achieved using recombinant clostridial acetogens supplied with syn(thesis) gas as the sole carbon and energy source. 3-HB production was successfully accomplished by a new synthetic pathway containing the genes thlA (encoding thiolase A), ctfA/B (encoding CoA-transferase A/B), and bdhA (encoding (R)-3-hydroxybutyrate dehydrogenase). The respective recombinant Clostridium coskatii [p83_tcb] strain produced autotrophically 0.98 ± 0.12 mM and heterotrophically 21.7 ± 0.27 mM 3-HB. As a proof of concept, production of PHB was achieved using recombinant C. coskatii and Clostridium ljungdahlii strains expressing a novel synthetic PHB pathway containing the genes thlA (encoding thiolase A), hbd (encoding 3-hydroxybutyryl-CoA dehydrogenase), crt (encoding crotonase), phaJ (encoding (R)-enoyl-CoA hydratase), and phaEC (encoding PHA synthase). The strain C. coskatii [p83_PHB_Scaceti] synthesized heterotrophically 3.4 ± 0.29% PHB per cell dry weight (CDW) and autotrophically 1.12 ± 0.12% PHB per CDW.


Mots-clés:
Type d'article:
scientifique
Faculté:
Ingénierie et Architecture
Ecole:
HEI-VS
Institut:
Institut Technologies du vivant
Date:
2019-05
Pagination:
12 p.
Publié dans:
Biomacromolecules
Numérotation (vol. no.):
2019, vol. 20, pp. 3271-3282
DOI:
ISSN:
1525-7797
Le document apparaît dans:

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 Notice créée le 2020-01-31, modifiée le 2020-10-27

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