Remedial Treatment of Corroded Iron Objects by Environmental Aeromonas Isolates

Kooli, Wafa M. (University of Neuchâtel, Switzerland) ; Junier, Thomas (University of Neuchâtel, Switzerland) ; Shakya, Migun (Los Alamos National Laboratory, New Mexico, USA) ; Monachon, Mathilde (University of Neuchâtel, Switerland) ; Davenport, Karen W. (Los Alamos National Laboratory, New Mexico, USA) ; Valdeeswaran, Kaushik (Centre Suisse d'Electronique et de Microtechnique, Neuchâtel, Switzerland) ; Vernudachi, Alexandre (NVENesis, Neuchâtel, Switzerland) ; Marozau, Ivan (Centre Suisse d'Electronique et de Microtechnique, Neuchâtel, Switzerland) ; Monrouzeau, Teddy (University of Neuchâtel, Switzerland) ; Gleasner, Cheryl D. (Los Alamos Laboratory, New Mexico, USA) ; McKurry, Kim (Los Alamos Laboratory, New Mexico, USA) ; Lienhard, Reto (ADMED Microbiologie, La Chaux-de-Fonds, Switzerland) ; Rufener, Lucien (NVENesis, Neuchâtel, Switzerland) ; Perret, Jean-Luc (NVENesis, Neuchâtel, Switzerland) ; Sereda, Olha (Centre Suisse d'Electronique et de Microtechnique, Neuchâtel, Switzerland) ; Chain, Patrick S. (Los Alamos Laboratory, New Mexico, USA) ; Joseph, Edith (Haute Ecole Arc Conservation-Restauration, HES-SO University of Applied Sciences and arts Western Switzerland) ; Junier, Pilar (University of Neuchâtel, Switzerland)

Using bacteria to transform reactive corrosion products into stable compounds represents an alternative to traditional methods employed in iron conservation. Two environmental Aeromonas strains (CA23 and CU5) were used to transform ferric iron corrosion products (goethite and lepidocrocite) into stable ferrous ironbearing minerals (vivianite and siderite). A genomic and transcriptomic approach was used to analyze the metabolic traits of these strains and to evaluate their pathogenic potential. Although genes involved in solid-phase iron reduction were identified, key genes present in other environmental iron-reducing species are missing from the genome of CU5. Several pathogenicity factors were identified in the genomes of both strains, but none of these was expressed under iron reduction conditions. Additional in vivo tests showed hemolytic and cytotoxic activities for strain CA23 but not for strain CU5. Both strains were easily inactivated using ethanol and heat. Nonetheless, given a lesser potential for a pathogenic lifestyle, CU5 is the most promising candidate for the development of a bio-based iron conservation method stabilizing iron corrosion. Based on all the results, a prototype treatment was established using archaeological items. On those, the conversion of reactive corrosion products and the formation of a homogenous layer of biogenic iron minerals were achieved. This study shows how naturally occurring microorganisms and their metabolic capabilities can be used to develop bio-inspired solutions to the problem of metal corrosion.

Article Type:
Design et Arts visuels
Unité de recherche en Conservation-restauration de la Haute Ecole Arc - UR-ARC CR
Conservation - Restauration
Published in:
Applied and environmental microbiology
Numeration (vol. no.):
2019, vol. 85, issue 3, e02042-18
Appears in Collection:

 Record created 2020-03-25, last modified 2020-10-27

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