Cold atmospheric plasma inactivation of microbial spores compared on reference surfaces and powder particles

Beyrer, Michael (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland) ; Smeu, Irina (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland ; National R&D Institute for Food Bioresources–IBA Bucharest, Bucharest, Romania) ; Martinet, David (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland) ; Howling, Alan (Swiss Plasma Center, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland) ; Pina-Perez, Maria Consuelo (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland) ; Ellert, Christoph (School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO // University of Applied Sciences Western Switzerland)

Heat-resistant spores on a dry, heat- and water-sensitive food matrix are difficult to inactivate. Radioactive or X-ray exposure is allowed and accepted only for some selected commodities. Non-thermal atmospheric pressure plasma treatments could offer an efficient, fast, and chemical-free solution. The effectiveness of direct contact cold atmospheric plasma (CAP) generated by a dielectric barrier discharge (DBD) device and air as process gas was evaluated against spores of Bacillus spp., Geobacillus spp., and Penicillium spp. A maximum of 3 log10 cycles of inactivation was achieved for B. coagulans spores exposed for only 10 s at low surface energy of 0.18 W/cm2 determined directly at the electrodes. This corresponds to an initial decimal reduction time of D1 = 0.1 min. Spores of B. subtilis are the most resistant amongst the studied strains (D1 = 1.4 min). The determining parameter in the modeling of the inactivation curve is surface energy. Non-porous, native starch granules or shells from diatoms, a highly porous material, were also contaminated with spores and treated by DBD CAP. The inactivation level was significantly reduced by the presence of powders. Considering plasma diagnostics, it can be concluded that the spore shell is the primary and main target for a plasma-induced inactivation. The inactivation affect scales with surface energy and can be controlled directly via process time and/or discharge power.


Keywords:
Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEI-VS
Institute:
Institut Systèmes industriels
Institut Technologies du vivant
Date:
2020-04
Pagination:
11 p.
Published in:
Food and Bioprocess Technology
Numeration (vol. no.):
2020, vol. 13, pp. 827-837
DOI:
ISSN:
1935-5130
Appears in Collection:



 Record created 2020-05-19, last modified 2020-05-20

Fulltext:
Download fulltext
PDF

Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)