Selection and mass production of santalum album l. calli for induction of sesquiterpenes

Crovadore, Julien (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Schalk, Michel (Firmenich S.A., Biotechnology Department, Corporate R&D Division, Geneva, Switzerland) ; Lefort, François (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland)

Young hypocotyl segments obtained from aseptically germinated seeds of Santalum album L. (5 weeks old) were used to induce callus formation. Growth of different calli types was induced on hypocotyls segments under different conditions. Resulting green calli were maintained on MS medium and Gamborg basal medium supplemented with 2,4D (2,4-Dichlorophenoxyacetic acid) (0.5 μM) and Kin (Kinetin) (10 μM). Numerous concentrations of 2,4D and Kin were tested in combination with inducers of the sesquiterpene metabolism and one promising callus type was selected for mass production. This interesting type of callus has been used for searching functional genes in the biosynthetic pathway leading to sesquiterpenes molecules.


Keywords:
Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEPIA - Genève
Institute:
inTNE - Institut Terre-Nature-Environnement
Date:
2012-02
Pagination:
5 p.
Published in:
Biotechnology & Biotechnological Equipment
Numeration (vol. no.):
2012, vol. 26, no. 2, pp. 2870-2874
DOI:
ISSN:
1310-2818
Appears in Collection:



 Record created 2020-08-13, last modified 2020-10-27

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