NIR-emitting and photo-thermal active nanogold as mitochondria-specific probes

Chakrabortty, Sabyasachi (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany) ; Sison, Miguel (EPFL, Lausanne, Switzerland) ; Wu, Yuzhou (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany) ; Ladenburger, Anita (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany) ; Pramanik, Goutam (Insitute of Organic Chemistry III, Ulm University, Ulm, Germany) ; Biskupek, Johannes (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany) ; Extermann, Jérôme (EPFL, Lausanne, Switzerland ; School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Kaiser, Ute (Central Facility of Electron Microscopy, University of Ulm, Ulm, Germany) ; Lasser, Theo (EPFL, Lausanne, Switzerland) ; Weil, Tanja (Institute of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany)

We report a bioinspired multifunctional albumin derived polypeptide coating comprising grafted poly(ethylene oxide) chains, multiple copies of the HIV TAT derived peptide enabling cellular uptake as well as mitochondria targeting triphenyl-phosphonium (TPP) groups. Exploring these polypeptide copolymers for passivating gold nanoparticles (Au NPs) yielded (i) NIR-emitting markers in confocal microscopy and (ii) photo-thermal active probes in optical coherence microscopy. We demonstrate the great potential of such multifunctional protein-derived biopolymer coatings for efficiently directing Au NP into cells and to subcellular targets to ultimately probe important cellular processes such as mitochondria dynamics and vitality inside living cells.


Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEPIA - Genève
Institute:
inSTI - Institut des Sciences et Technologies industrielles
Date:
2017-02
Pagination:
10 p.
Published in:
Biomaterials Science
Numeration (vol. no.):
2017, vol. 5, no. 5, article no. 966-971
DOI:
ISSN:
2047-4830
Appears in Collection:



 Record created 2020-09-15, last modified 2021-02-23

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