3D time-lapse imaging and quantification of mitochondrial dynamics

Sison, Miguel (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland) ; Chakrabortty, Sabyasachi (Department of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany) ; Extermann, Jérôme (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland ; School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Nahas, Amir (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland) ; Marchand, james Paul (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland) ; Lopez, Antonio (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland) ; Weil, Tanja (Department of Organic Chemistry III, Ulm University, Ulm, Germany ; Max-Planck-Institute for Polymer Research, Mainz, Germany) ; Lasser, Theo (Laboratoire d'Optique Biomédicale, EPFL, Lausanne, Switzerland)

We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake and mitochondria targeting efficiency. The high stability of the gold nanoparticles allows continuous imaging over an extended time up to 3000 seconds without significant cell damage. By combining temporal autocorrelation analysis with a classical diffusion model, we quantify mitochondrial dynamics and cast these results into 3D maps showing the heterogeneity of diffusion parameters across the whole cell volume.


Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEPIA - Genève
Institute:
inSTI - Institut des Sciences et Technologies industrielles
Date:
2017-02
Pagination:
8 p.
Published in:
Scientific Reports
Numeration (vol. no.):
2017, vol. 7, article no. 43275
DOI:
ISSN:
2045-2322
Appears in Collection:



 Record created 2020-09-15, last modified 2020-10-27

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