High resolution optical projection tomography platform for multispectral imaging of the mouse gut

Schmidt, Cédric (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Planchette, Arielle L. (EPFL, Lausanne, Switzerland) ; Nguyen, David (Zlatic Lab, Neurobiology, MRC-Laboratory of Molecular Biology, Cambridge, United Kingdom) ; Giardina, Gabriel (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Neuenschwander, Yoan (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Di Franco, Mathieu (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Mylonas, Alessio (EPFL, Lausanne, Switzerland) ; Descloux, Adrien C. (EPFL, Lausanne, Switzerland) ; Pomarico, Enrico (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland) ; Radenovic, Aleksandra (EPFL, Lausanne, Switzerland) ; Extermann, Jérôme (School of Engineering, Architecture and Landscape (hepia), HES-SO // University of Applied Sciences Western Switzerland)

Optical projection tomography (OPT) is a powerful tool for three-dimensional imaging of mesoscopic biological samples with great use for biomedical phenotyping studies. We present a fluorescent OPT platform that enables direct visualization of biological specimens and processes at a centimeter scale with high spatial resolution, as well as fast data throughput and reconstruction. We demonstrate nearly isotropic sub-28 μm resolution over more than 60 mm3 after reconstruction of a single acquisition. Our setup is optimized for imaging the mouse gut at multiple wavelengths. Thanks to a new sample preparation protocol specifically developed for gut specimens, we can observe the spatial arrangement of the intestinal villi and the vasculature network of a 3-cm long healthy mouse gut. Besides the blood vessel network surrounding the gastrointestinal tract, we observe traces of vasculature at the villi ends close to the lumen. The combination of rapid acquisition and a large field of view with high spatial resolution in 3D mesoscopic imaging holds an invaluable potential for gastrointestinal pathology research.


Article Type:
scientifique
Faculty:
Ingénierie et Architecture
School:
HEPIA - Genève
Institute:
inSTI - Institut des Sciences et Technologies industrielles
Date:
2021-06
Pagination:
11 p.
Published in:
Biomedical Optics Express
Numeration (vol. no.):
2021, vol. 12, no. 6, pp. 3619-3629
DOI:
ISSN:
2156-7085
Appears in Collection:



 Record created 2021-06-29, last modified 2021-06-29

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