The current study presents the CO2-eq emissions of the operational energy use of a single-family house, equipped with a micro-cogeneration unit. A back-up boiler and electricity from the grid cover the remaining energy demand, not covered by the micro-CHP. Two different technologies are evaluated, i.e. ICE and fuel cell systems, operating with a variable share of biomethane, while two different substrates were considered for the biomethane generation. A dynamic LCA was applied for the electricity mix, coming from the grid, using different time steps. The results show that producing biomethane from biowaste compared to conventional natural gas is beneficial, in terms of CO2-eq emissions, independently of the micro-CHP technology, while the total CO2-eq emissions of the fuel cell technology are higher than those of the ICE, independently of the substrate and the biomethane share.