Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro
Schmid, Sergio; Berger, Bernard; Haas, Dieter
1999
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Résumé

Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage λ in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.

Détails

Titre Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro
Auteur(s)/ trice(s) Schmid, Sergio (ETH Zürich, Zürich, Switzerland ; School of Engineering, HES-SO Valais-Wallis, HEI, HES-SO University of Applied Sciences Western Switzerland)
Berger, Bernard (Université de Lausanne, Lausanne, Switzerland)
Haas, Dieter (ETH Zürich, Zürich, Switzerland ; Université de Lausanne, Lausanne, Switzerland)
Date 1999-04
Publié dans Journal of Bacteriology
Volume 1999, vol. 181, no. 7, pp. 2286-2289
Pagination 4 p.
DOI https://doi.org/10.1128/JB.181.7.2286-2289.1999
ISSN 0021-9193
Type d'article scientifique
Domaine Ingénierie et Architecture
Ecole HEI-VS
Institut Institut Technologies du vivant
Le document apparaît dans Articles scientifiques
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